Place the axis in vertical position.
Active air sampling sop.
Airborne gases and vapours are collected by a physical process such as diffusion through a static air layer or permeation through a membrane.
5 9 7 close the media cassettes properly after sampling 5 9 8 clean the pump with 70 ipa before taking it to the next sampling location port.
Analyse the microbial quality of air by sampling 100 litre of air at each of the locations using active air sampler.
At 3 cm of the end of the axis screw a nut.
Typically sampled air is drawn by a suction pump through a narrow inlet tube into a small.
Active sampling involves the use of an air sampling pump to actively pull air through a collection device such as a filter.
Analyze the microbial quality of air by sampling 100 litres of air at each of the locations mentioned in annexure i using active air sampler carry the pre incubated scda plates and sterile ss head in a ss container to the sampling location.
6 2 switching the sampler on.
Fertility test of media.
When the pre set sampling cycle is completed the plate is removed and incubated.
5 9 5 regulate the pressure of the air so that the flow rate is in between 140l to 180l per minute.
1 impingers impingers use a liquid medium for particle collection.
Active air sampling sites should be done at the same locations or as close as practical to avoid compro mising the other measure or the product integrity.
Carry the preincubated scda plates and sterile ss head in a ss container to the sampling location.
Active and passive monitoring.
The organisms are then visible to the naked eyes and can be counted for an assessment of the level of contamination.
6 2 2 after pressing the on off button the previously selected air sample volume retained in the memory appears on the display.
In active monitoring a microbial air sampler is used to force air into or onto its collection medium e g petri dish with nutrient agar based test media over a specified period of time.
This leaves determination of the number ofsites for passive air sampling and surface sampling.
6 2 1 push the on off button.
Perform the growth promotion test of each lot of media used for environmental monitoring as per sop.
Active there are two primary methods for microbial air sampling.
5 2 operation of air.
Enter into the aseptic area following aseptic area entry and exit sop of the concerned block.
Active air sampling requires the use of a microbiology air sampler to physically draw a known volume of air over or through a particle collection device and there are two main types.
Passive air sampling passive air sampling i e settle plates is a frequently.
Standard operating procedures for the passive air sampling 2 2 pas assembling the pas should be assembled from the bottom to the top.
The following steps describe the way to assemble the passive air sampler.
Passive sampling however does not require active air movement from a pump.
Sterilise the stainless steel aspirating head of the air sampler prior to use by autoclaving as per sop.
5 9 6 ensure that m air t media cassette is in place before sampling is started.