Active Air Sampling Procedure

In case the active air sampler is out of order then settle plate method can be used as alternative for monitoring the microbiological quality of air.

Active air sampling procedure.

This procedure is applicable for the deployment of pas in urban suburban rural and remote areas. Active air sampling requires the use of a microbiology air sampler to physically draw a known volume of air over or through a particle collection device and there are two main types. Procedure for air sampling in pharmaceuticals 1 prepare scda medium and aseptically pour approximately 15 20 ml of sterile molten cooled 40 c scda agar into sterile 90 mm petri plates 2 allow solidifying the plates at room temp after solidification label all the plates with the name of media preparation batch no. Analyze the microbial quality of air by sampling 100 litres of air at each of the locations mentioned in annexure i using active air sampler carry the pre incubated scda plates and sterile ss head in a ss container to the sampling location.

And date of preparation. Active there are two primary methods for microbial air sampling. As i mentioned above in active air sampling we determine bioburden in 1 cubic meter area and we sample 1000 liters of air by air sampler while in passive air sampling we determine that how much microbes settle in 90 mm diameter surface of any equipment exposed in controlled area. In active monitoring a microbial air sampler is used to force air into or onto its collection medium e g petri dish with nutrient agar based test media over a specified period of time.

Procedure the microbial environmental conditions of unit ii shall be monitored by settle plate method or air sampling at the locations. Active sampling is a microbial sampling monitoring technique whereby an air sampling device or pump is used to force air into or onto a collection medium for example petri dish with nutrient. Passive sampling however does not require active air movement from a pump. Airborne gases and vapours are collected by a physical process such as diffusion through a static air layer or permeation through a membrane.

Active and passive monitoring. The objective of this procedure is to describe the management of air passive samplers pas for sampling pollutants including the main steps for assembling and disassembling as well as maintenance of the passive air samplers.